Caspase-9 and APAF-1 form an active holoenzyme
نویسندگان
چکیده
منابع مشابه
Caspase-9 and APAF-1 form an active holoenzyme.
Autocatalytic activation of initiator caspases is the link between pro-apoptotic signals and the destruction machinery of apoptosis. Activation of caspase-9, which mediates oncogene and drug-induced apoptosis, requires binding to the protein APAF-1. We found that the proteolytic activity of caspase-9 in a complex with APAF-1 is several orders of magnitude higher than that of the free enzyme. Th...
متن کاملReconstitution and assay of the activity of the caspase-9±Apaf-1 holoenzyme complexes
Recombinant WT and mutant caspase-9 proteins (20 nM) were incubated in the presence or absence of recombinant Apaf-1 (20 nM) in buffer A. The reaction mixtures were stimulated with cytochrome c (5 ng ml) and dATP (1 mM) and incubated for 0±60 min at 30 8C with puri®ed procaspase-3 C163A, and the processing of procaspase-3 was analysed by western blot analysis with anti-caspase-3 antibody (Fig. ...
متن کاملRegulation of the Apaf-1-caspase-9 apoptosome.
caspase-9 apoptosome Shawn B. Bratton1 and Guy S. Salvesen2 1Division of Pharmacology and Toxicology, College of Pharmacy, and Center for Molecular and Cellular Toxicology, and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712-0125, USA 2Program in Apoptosis and Cell Death Research, Sanford-Burnham Medical Research Institute, 10901 North Torrey Pi...
متن کاملMaturation of the olfactory sensory neurons by Apaf-1/caspase-9-mediated caspase activity.
Although the apoptotic role of caspases has been largely understood, accumulating evidence in Drosophila suggests that caspases also control other processes than apoptotic cell death. However, how caspases contribute to the development of the mammalian nervous system remains obscure. Here, we provide unique evidence that Apaf-1/caspase-9-mediated caspase signaling regulates the development of o...
متن کاملThe Apaf-1 apoptosome induces formation of caspase-9 homo- and heterodimers with distinct activities
According to dogma, initiator caspases are activated through proximity-induced homodimerization, but some studies infer that during apoptosis caspase-9 may instead form a holoenzyme with the Apaf-1 apoptosome. Using several biochemical approaches, including a novel site-specific crosslinking technique, we provide the first direct evidence that procaspase-9 homodimerizes within the apoptosome, m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genes & Development
سال: 1999
ISSN: 0890-9369
DOI: 10.1101/gad.13.24.3179